Get Closer to the Truth – Confocal Microscope Platform STELLARIS

STELLARIS has been designed from the ground up to give you the power to see more. The synergy between the new Power HyD detector family, the completely optimized beam path and the next generation White Light Lasers (WLL) empowers you to obtain more accurate and reliable data to test your hypothesis with precision.

Gain the potential to explore a new dimension in your samples using our new and unique TauSense technology. TauSense gives instant access to lifetime-based information, delivering additional insights to your experiments.

STELLARIS gives you increased productivity to do more thanks to our new smart user interface, ImageCompass, which enables you to acquire images with a few clicks, even for complex experiments.

STELLARIS 5

STELLARIS 5 delivers the very best of our platform. It is a completely re-imagined core system that sets a new standard for confocal microscopy. It is the only confocal system with an integrated WLL, combined with our proprietary Acousto-Optical Beam Splitter (AOBS) and new Power HyD S detectors. Together with the new and unique TauSense technology, STELLARIS 5 sets a new standard for the quality of images and quantity of information generated. This perfected imaging performance is easily attainable thanks to the smart user interface, ImageCompass, which guides you through your experiment set up and acquisition in an easy and intuitive manner.

STELLARIS 8

STELLARIS 8 is a forward-looking system, offering all the benefits of the STELLARIS 5 core system plus added functionality thanks to an extended spectrum WLL and specialized detector options of the Power HyD family. This allows you to expand the range of confocal applications in your research. STELLARIS 8 can be combined with all Leica Microsystems modalities, including FAst Lifetime CONtrast (FALCON), Deep In Vivo Explorer (DIVE), STED, Digital Light Sheet (DLS) and CARS. STELLARIS 8 new features maximize the potential of these modalities and give you the power and potential to set new standards for research.

Power HyD from 440nm up to 790nm Live image Contrast Image Compass LIGHTNING detection

The power to see more

Experience enhanced image quality thanks to the Leica Power HyD family ultrasensitive detection capacity. Our new detector family provide higher photon detection efficiency (PDE)* and extremely low dark noise, enabling you to image with more brightness and more detail.

Leica Microsystems new, proprietary approach to photon counting, Power Counting significantly improves image contrast, so you can obtain quantitative results from your confocal imaging experiments.

The unique combination of WLL, our unique AOBS, and our Power HyD detector family enables tuning excitation and detection to perfectly match the spectral profile of the dyes in your sample, giving you clear, sharp images.

* 2x higher Photon Detection Efficiency (PDE) compared to conventional Multi-Alkali Photomultiplier Tubes (PMT) and 3x higher in the extended red range

Maximized flexibility for multicolor experiments

Experience complete spectral freedom with STELLARIS. The STELLARIS next-generation WLL , paired with our Power HyD family of detectors, gives you complete spectral freedom to choose and combine exactly the right probes to answer your experimental questions. With up to 8 simultaneous laser lines available to choose from 440 nm up to 790 nm, you have the capacity to image more fluorophore combinations than any other confocal platform. All of this with less complexity and more flexibility from a single laser doing the work of many.

Image more markers within one sample at the same time. STELLARIS has been optimized with next generation WLL with an extended spectral output on the red and near-infrared spectrum up to 790 nm. This allows you to expand your experiment with up to 3 more colors without increasing experimental complexity.

Gentle live cell imaging

The goal of any experiment with a live specimen is to make detailed observations without influencing or damaging your sample. STELLARIS enables you to image for longer periods preserving physiological conditions and sample integrity thanks to the optimally tuned excitation and detection provided by the Power HyD detector family and WLL combination, since both excitation and detection are optimally tuned.

The high detection efficiency of our Power HyD detector family in the green/blue segment of the spectrum provides you with improved imaging capabilities for the most used fluorescent proteins, such as GFP or YFP, thanks to efficient signal acquisition at the lowest levels of illumination.

With STELLARIS you don’t need to compromise sample health to get great images. Whether you need long acquisition times for high-resolution 3D reconstructions or the highest frame rates to capture rapid dynamic events, our efficient signal detection ensures that you can perform the experiments that you need, while your samples are protected from photobleaching and phototoxicity.

TauSense

TauSense is a set of imaging tools based on fluorescence lifetime, a form of contrast independent from fluorescence intensity. These imaging tools include TauContrast, TauGating and TauSeparation, which can help you understand the function of molecules within the cellular environment, increase the quality of your images, and expand the number of probes that you can study in your sample. The potential is there, sitting in your samples and waiting to be found.

ImageCompass – Simple, even for complex experiments

Software should not hold you back when using your confocal microscope; it should make life easier. Our newly developed smart user interface ImageCompass makes the setting up complex experiments significantly easier.

ImageCompass features a drag-and-drop menu to add fluorophores and guides you through the setup multicolor experiments. It also has to ability to perform automated optimization of the excitation and detection parameters to give you maximum signal yield.

ImageCompass moves technical parameters to the background, leaving practical information in prominent positions and giving you a cockpit view of your experiment, so you always have the information you need to stay in control of your experiment.

With ImageCompass you also get intuitive access to functional information from TauSense and super-resolution with LIGHTNING. ImageCompass saves you time and adapts to your sample preparation, leaving you to focus on your experiment and giving you the productivity to do more.

Fast across scales in time and space

LIGHTNING detection technology is fully integrated in STELLARIS for fast super-resolution confocal imaging in real time. LIGHTNING automatically detects the finest structures and details using an adaptive process for extraction of hidden information. Unlike traditional technologies that use a global set of parameters for the full image, LIGHTNING calculates an appropriate set of parameters for each voxel to uncover every detail with the highest fidelity.

With STELLARIS, you have direct access to the resonant scanner, which you can activate to improve image quality while preserving temporal resolution. Fast processing from LIGHTNING coupled with our resonant scanner enables high-resolution scans over a large area to produce exceptional image quality in record time.

Additionally, using the resonant scanner in combination with our newly implemented Dynamic Signal Enhancement enables you to adapt to your sample’s dynamics to get the best image resolution.

Get Closer to the Truth – Confocal Microscope Platform STELLARIS

STELLARIS 5

STELLARIS 8

The power to see more

Maximized flexibility for multicolor experiments

Gentle live cell imaging

TauSense

ImageCompass – Simple, even for complex experiments

Fast across scales in time and space

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